Catalog Number - 68504-15P-2

Cogent RP C4™ is a reversed-phase HPLC stationary phase designed for the separation of hydrophobic and neutral compounds where less retention is desired than with traditional C8 or C18 phases. Manufactured using a four-carbon butyl ligand bonded to ultra-high purity silica and fully double end capped, this stationary phase provides reproducible chromatographic performance and is widely used as an alternative in USP methods requiring L26 chemistry. Its reduced hydrophobicity compared to longer-chain bonded phases makes it particularly useful for rapid analyses, routine testing, and method development.

68504-15P-2 is an HPLC column packed with 5 µm RP C4™ particles featuring a 100 Å pore size in a 2.1 mm ID x 150 mm format. This narrow-bore configuration combines excellent chromatographic resolution with reduced solvent consumption and compatibility with LC-MS instrumentation. The 150 mm length provides increased peak capacity and resolving power compared to shorter column formats, while the 5 µm particles provide robust performance with moderate backpressure. Typical operating flow rates are approximately 0.2–0.3 mL/min. Standard HPLC systems used with 2.1 mm ID columns must be capable of precise and reproducible low-flow operation.

This column is commonly used for hydrophobic and neutral compounds in reversed-phase HPLC when reduced retention is desirable. Compared to C8 phases, RP C4™ typically provides lower retention, making it useful for compounds that may otherwise be excessively retained on more hydrophobic stationary phases. The 2.1 mm ID format is especially popular for LC-MS methods due to its low solvent consumption, enhanced sensitivity, and efficient analyte transfer to the mass spectrometer.

Key Benefits

• RP C4™ stationary phase

• USP L26 equivalent chemistry

• Butyl bonded phase

• Fully double end capped

• Ultra-high purity silica support

• Less retention than C8 phases

• Suitable for rapid analyses

• Reduced solvent consumption

• 5 µm particle size

• Excellent for LC-MS applications

Typical Applications

• USP L26 methods

• LC-MS methods

• Method development

• Method scouting

• Hydrophobic compound analysis

• Neutral compound analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Quality control testing

• Research and development

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C4™

• USP Classification: L26

• Chromatography Mode: Reversed Phase

• Bonded Phase: Butyl (C4)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 150 mm

• Length: 150 mm

• Typical Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for hydrophobic and neutral compounds

• Provides less retention than C8 stationary phases

• Suitable for rapid reversed-phase separations

• The 2.1 mm ID format is commonly used for LC-MS applications

• Standard HPLC systems must be capable of reliable operation at approximately 0.2–0.3 mL/min

• Detector flow cells should be optimized for low-volume, narrow-bore chromatography

• The 150 mm length provides greater resolution and peak capacity than shorter column formats

• 5 µm particles provide robust performance with moderate operating pressures

• Fully double end capped to minimize secondary interactions

• Frequently used for method development and scouting studies